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Discuss John McCann's answer to: Degradation of Gst-fusion proteins following IPTG induction in E.coli.

Gst Fusion protein shows degradation following IPTG induction. The recombinant pGEX/4T1 plasmid was used to transform BL21(DE3)pLysE cells. Individual colonies were cultured, and fusion protein ...

John McCann
John McCann Answered:

Hardly! You need a biotechnologist familiar as hell with Western blot technique and possible an immunologist very familiar with quantifying proteins.

" Reality is that which exists outside of your beliefs. "
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